recombinant mouse probdnf Search Results


recombinant mouse cleavage resistant probdnf  (Alomone Labs)
93
Alomone Labs recombinant mouse cleavage resistant probdnf
A) Male and female ChAT.eGFP mice were allocated into one of five groups: i) PBS (control); ii) CNTF; iii) <t>proBDNF;</t> iv) HGF; and v) NTRN. B) Each mouse received intramuscular injections of H C T combined with a single NTF into the left tibialis anterior and right soleus muscles to target fast and slow motor neurons, respectively. After a 4-8 h incubation period, time-lapse microscopy was performed on both sciatic nerves. C) H C T-labelled signalling endosomes (pseudo-coloured in magenta) from single ChAT.eGFP motor axons were individually tracked to quantify retrograde transport dynamics. Three representative retrogradely transported signalling endosomes are identified by yellow, cyan, and peach arrowheads connected by dashed lines across frames. Grey arrowheads and dashed lines identify a stationary endosome. See also Video 1 . Scale bar = 5 μm, frame interval = 3 s.
Recombinant Mouse Cleavage Resistant Probdnf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+probdnf/bio_rxiv__2025__10__09__681259-48-37-41?v=Alomone+Labs
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probdnf  (Alomone Labs)
90
Alomone Labs probdnf
<t>ProBDNF</t> inhibits PF in entorhinal pyramidal neurons. Current-clamp recording from pyramidal neurons in layer V EC. A, Dose-dependent induction of PF by CCh in control condition: CCh at 10 μm (10 min), but not 5 μm (10 min), induces PF. B, Quantification of the plateau amplitude and the frequency of A (CCh: 5 μm, n = 16; 10 μm, n = 8). C, D, 10 min application of proBDNF at 2 ng/ml blocks PF in wild-type mice (C, WT, n = 5) but not <t>in</t> <t>p75NTR−/−</t> mice (D, n = 5). E, F, Quantification of the plateau amplitude (E) and the frequency (F) of C and D.
Probdnf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+probdnf/pmc06605143-283-18-23?v=Alomone+Labs
Average 90 stars, based on 1 article reviews
probdnf - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier

Image Search Results


A) Male and female ChAT.eGFP mice were allocated into one of five groups: i) PBS (control); ii) CNTF; iii) proBDNF; iv) HGF; and v) NTRN. B) Each mouse received intramuscular injections of H C T combined with a single NTF into the left tibialis anterior and right soleus muscles to target fast and slow motor neurons, respectively. After a 4-8 h incubation period, time-lapse microscopy was performed on both sciatic nerves. C) H C T-labelled signalling endosomes (pseudo-coloured in magenta) from single ChAT.eGFP motor axons were individually tracked to quantify retrograde transport dynamics. Three representative retrogradely transported signalling endosomes are identified by yellow, cyan, and peach arrowheads connected by dashed lines across frames. Grey arrowheads and dashed lines identify a stationary endosome. See also Video 1 . Scale bar = 5 μm, frame interval = 3 s.

Journal: bioRxiv

Article Title: CNTF specifically slows down the axonal transport of signalling endosomes

doi: 10.1101/2025.10.09.681259

Figure Lengend Snippet: A) Male and female ChAT.eGFP mice were allocated into one of five groups: i) PBS (control); ii) CNTF; iii) proBDNF; iv) HGF; and v) NTRN. B) Each mouse received intramuscular injections of H C T combined with a single NTF into the left tibialis anterior and right soleus muscles to target fast and slow motor neurons, respectively. After a 4-8 h incubation period, time-lapse microscopy was performed on both sciatic nerves. C) H C T-labelled signalling endosomes (pseudo-coloured in magenta) from single ChAT.eGFP motor axons were individually tracked to quantify retrograde transport dynamics. Three representative retrogradely transported signalling endosomes are identified by yellow, cyan, and peach arrowheads connected by dashed lines across frames. Grey arrowheads and dashed lines identify a stationary endosome. See also Video 1 . Scale bar = 5 μm, frame interval = 3 s.

Article Snippet: Mice were divided into five experimental cohorts, and received 5-7 μg of H C T mixed with: a) phosphate buffered saline (PBS) as the control; b) 50 ng recombinant human CNTF protein (Peprotech, 450-13), c) 50 ng recombinant mouse cleavage-resistant proBDNF (Alomone labs, B-243), d) 25 ng recombinant human HGF (Bio-Techne, 294-HG[CF]), or e) 50 ng recombinant human NTRN (Peprotech, 450-11), as summarised in .

Techniques: Control, Muscles, Incubation, Time-lapse Microscopy

In both fast motor neurons (FMNs) and slow motor neurons (SMNs), proBDNF did not alter A) mean endosome speed ( p = 0.06 for motor neuron type; p = 0.19 for stimulation factor; p = 0.70 for interaction), B) maximum endosome speed ( p = 0.30 for motor neuron type; p = 0.62 for stimulation factor; p = 0.25 for interaction) or C) pausing percentage ( p = 0.24 for motor neuron type; p = 0.92 for stimulation factor; p = 0.34 for interaction). D ) Violin plots of individual endosomes show comparable distributions in all conditions (FMNs: PBS mean = 2.78 µm/s ± 0.03 [n = 495], proBDNF mean = 2.67 µm/s ± 0.03 [n = 496]; SMNs: PBS mean = 2.57 µm/ s ± 0.03 [n = 495], proBDNF mean = 2.53 µm/s ± 0.03 [n = 477]). Overlapping endosome frame-to-frame and mean endosome speed distribution curves confirm that proBDNF does not modulate retrograde transport in E) FMNs or F) SMNs. Statistical analyses were performed using two-way ANOVA and Holm-Šídák 1 s multiple comparisons tests. ns, not significant. n = 7-8. Black circles = males (n = 4 PBS; n = 2 CNTF), white circles = females (n = 4 PBS; n = 5 proBDNF).

Journal: bioRxiv

Article Title: CNTF specifically slows down the axonal transport of signalling endosomes

doi: 10.1101/2025.10.09.681259

Figure Lengend Snippet: In both fast motor neurons (FMNs) and slow motor neurons (SMNs), proBDNF did not alter A) mean endosome speed ( p = 0.06 for motor neuron type; p = 0.19 for stimulation factor; p = 0.70 for interaction), B) maximum endosome speed ( p = 0.30 for motor neuron type; p = 0.62 for stimulation factor; p = 0.25 for interaction) or C) pausing percentage ( p = 0.24 for motor neuron type; p = 0.92 for stimulation factor; p = 0.34 for interaction). D ) Violin plots of individual endosomes show comparable distributions in all conditions (FMNs: PBS mean = 2.78 µm/s ± 0.03 [n = 495], proBDNF mean = 2.67 µm/s ± 0.03 [n = 496]; SMNs: PBS mean = 2.57 µm/ s ± 0.03 [n = 495], proBDNF mean = 2.53 µm/s ± 0.03 [n = 477]). Overlapping endosome frame-to-frame and mean endosome speed distribution curves confirm that proBDNF does not modulate retrograde transport in E) FMNs or F) SMNs. Statistical analyses were performed using two-way ANOVA and Holm-Šídák 1 s multiple comparisons tests. ns, not significant. n = 7-8. Black circles = males (n = 4 PBS; n = 2 CNTF), white circles = females (n = 4 PBS; n = 5 proBDNF).

Article Snippet: Mice were divided into five experimental cohorts, and received 5-7 μg of H C T mixed with: a) phosphate buffered saline (PBS) as the control; b) 50 ng recombinant human CNTF protein (Peprotech, 450-13), c) 50 ng recombinant mouse cleavage-resistant proBDNF (Alomone labs, B-243), d) 25 ng recombinant human HGF (Bio-Techne, 294-HG[CF]), or e) 50 ng recombinant human NTRN (Peprotech, 450-11), as summarised in .

Techniques:

ProBDNF inhibits PF in entorhinal pyramidal neurons. Current-clamp recording from pyramidal neurons in layer V EC. A, Dose-dependent induction of PF by CCh in control condition: CCh at 10 μm (10 min), but not 5 μm (10 min), induces PF. B, Quantification of the plateau amplitude and the frequency of A (CCh: 5 μm, n = 16; 10 μm, n = 8). C, D, 10 min application of proBDNF at 2 ng/ml blocks PF in wild-type mice (C, WT, n = 5) but not in p75NTR−/− mice (D, n = 5). E, F, Quantification of the plateau amplitude (E) and the frequency (F) of C and D.

Journal: The Journal of Neuroscience

Article Title: proBDNF and p75NTR Control Excitability and Persistent Firing of Cortical Pyramidal Neurons

doi: 10.1523/JNEUROSCI.4655-14.2015

Figure Lengend Snippet: ProBDNF inhibits PF in entorhinal pyramidal neurons. Current-clamp recording from pyramidal neurons in layer V EC. A, Dose-dependent induction of PF by CCh in control condition: CCh at 10 μm (10 min), but not 5 μm (10 min), induces PF. B, Quantification of the plateau amplitude and the frequency of A (CCh: 5 μm, n = 16; 10 μm, n = 8). C, D, 10 min application of proBDNF at 2 ng/ml blocks PF in wild-type mice (C, WT, n = 5) but not in p75NTR−/− mice (D, n = 5). E, F, Quantification of the plateau amplitude (E) and the frequency (F) of C and D.

Article Snippet: All drugs were purchased from Sigma-Aldrich, except 6-methyl-2-(phenylethynyl) pyridine, (S)-3,5-dihydroxyphenylglycine hydrate, and NSC23766 from Tocris Bioscience, mBDNF and proBDNF (wild-type mouse, #B240) from Alomone Labs, p75NTR antibody (AB-N01) from Advanced Targeting Systems, and proBDNF antibody (5H8) from Santa Cruz Biotechnology.

Techniques:

mBDNF does not block the effect of proBDNF on persistent firing. A, BDNF (10 min, 2 ng/ml) does not inhibit PF. B, Quantification of the plateau amplitude and the frequency of A (n = 4). C, PF is blocked in slices exposed to a combination of proBDNF [at 2 ng/ml (38 pm)] and BDNF [at 10.3 ng/ml (381 pm); n = 5]. D, Quantification of plateau amplitude and firing frequency from C. *p < 0.05, Student's t test.

Journal: The Journal of Neuroscience

Article Title: proBDNF and p75NTR Control Excitability and Persistent Firing of Cortical Pyramidal Neurons

doi: 10.1523/JNEUROSCI.4655-14.2015

Figure Lengend Snippet: mBDNF does not block the effect of proBDNF on persistent firing. A, BDNF (10 min, 2 ng/ml) does not inhibit PF. B, Quantification of the plateau amplitude and the frequency of A (n = 4). C, PF is blocked in slices exposed to a combination of proBDNF [at 2 ng/ml (38 pm)] and BDNF [at 10.3 ng/ml (381 pm); n = 5]. D, Quantification of plateau amplitude and firing frequency from C. *p < 0.05, Student's t test.

Article Snippet: All drugs were purchased from Sigma-Aldrich, except 6-methyl-2-(phenylethynyl) pyridine, (S)-3,5-dihydroxyphenylglycine hydrate, and NSC23766 from Tocris Bioscience, mBDNF and proBDNF (wild-type mouse, #B240) from Alomone Labs, p75NTR antibody (AB-N01) from Advanced Targeting Systems, and proBDNF antibody (5H8) from Santa Cruz Biotechnology.

Techniques: Blocking Assay

ProBDNF inhibits cholinergic calcium responses and PF via a Rac1-PIP2 pathway. A, Normalized Fura-2 responses of cortical neurons treated with vehicle (n = 55) or proBDNF 2 ng/ml (n = 68) 10 min and then subject to CCh (100 μm) for 1 min. B, Area under curve of A. ***p = 0.0006, t test. C, Current-clamp recording from pyramidal neuron in wild-type mice; 30 min with LY294002 20 μm within the patch pipette avoid the cell to fire again. D, proBDNF blocks PF (Fig. 1E). E, Wortmannin (20 μm, 15 min) within the patch-pipette solution blocks the effect of the proBDNF. F, The Rac1 inhibitor NSC23766 (50 μm, 15 min) in the patch-pipette solution blocks the effect of the proBDNF. G, Quantification of the plateau amplitude of D–F. H, Quantification of the frequency of D–F. *p < 0.05, one-way ANOVA followed by a Bonferroni's test.

Journal: The Journal of Neuroscience

Article Title: proBDNF and p75NTR Control Excitability and Persistent Firing of Cortical Pyramidal Neurons

doi: 10.1523/JNEUROSCI.4655-14.2015

Figure Lengend Snippet: ProBDNF inhibits cholinergic calcium responses and PF via a Rac1-PIP2 pathway. A, Normalized Fura-2 responses of cortical neurons treated with vehicle (n = 55) or proBDNF 2 ng/ml (n = 68) 10 min and then subject to CCh (100 μm) for 1 min. B, Area under curve of A. ***p = 0.0006, t test. C, Current-clamp recording from pyramidal neuron in wild-type mice; 30 min with LY294002 20 μm within the patch pipette avoid the cell to fire again. D, proBDNF blocks PF (Fig. 1E). E, Wortmannin (20 μm, 15 min) within the patch-pipette solution blocks the effect of the proBDNF. F, The Rac1 inhibitor NSC23766 (50 μm, 15 min) in the patch-pipette solution blocks the effect of the proBDNF. G, Quantification of the plateau amplitude of D–F. H, Quantification of the frequency of D–F. *p < 0.05, one-way ANOVA followed by a Bonferroni's test.

Article Snippet: All drugs were purchased from Sigma-Aldrich, except 6-methyl-2-(phenylethynyl) pyridine, (S)-3,5-dihydroxyphenylglycine hydrate, and NSC23766 from Tocris Bioscience, mBDNF and proBDNF (wild-type mouse, #B240) from Alomone Labs, p75NTR antibody (AB-N01) from Advanced Targeting Systems, and proBDNF antibody (5H8) from Santa Cruz Biotechnology.

Techniques: Transferring

proBDNF increases PIP2 at the plasma membrane of cortical neurons via a p75NTR-Rac1 pathway. The surface level of PIP2 was monitored in live primary cultures of cortical neurons transfected with the GFP-PLCΔ-PH reporter and incubated with rhodamine-labeled wheat-germ agglutinin. A, Representative images of control condition where neurons were treated with CCh (100 μm) for 20 min. Pictures were taken before application of CCh (top), after 10 min application of CCh (middle), and after 20 min application of CCh (bottom). B, Quantification of A (n = 6) after normalization to the GFP fluorescence at the membrane before drug application (see also Material and Methods). *p < 0.05, paired t test. White asterisks represent areas of membrane where the GFP fluorescence disappeared after application of CCh. C, Same experiment as in A but after initial 10 min of CCh, proBDNF (2 ng/ml) was coapplied with CCh for subsequent 10 min. D, Quantification of C (n = 7). E, Same experiment as in C but dishes were pretreated 2 h with a p75NTR function-blocking antibody (1:500). F, Quantification of E (n = 8). **p < 0.01 paired t test. G, Same experiment than in C but the Rac1 inhibitor NSC23766 (50 μm) was bath-applied throughout the experiment. H, Quantification of G (n = 5). Scale bar, 10 μm.

Journal: The Journal of Neuroscience

Article Title: proBDNF and p75NTR Control Excitability and Persistent Firing of Cortical Pyramidal Neurons

doi: 10.1523/JNEUROSCI.4655-14.2015

Figure Lengend Snippet: proBDNF increases PIP2 at the plasma membrane of cortical neurons via a p75NTR-Rac1 pathway. The surface level of PIP2 was monitored in live primary cultures of cortical neurons transfected with the GFP-PLCΔ-PH reporter and incubated with rhodamine-labeled wheat-germ agglutinin. A, Representative images of control condition where neurons were treated with CCh (100 μm) for 20 min. Pictures were taken before application of CCh (top), after 10 min application of CCh (middle), and after 20 min application of CCh (bottom). B, Quantification of A (n = 6) after normalization to the GFP fluorescence at the membrane before drug application (see also Material and Methods). *p < 0.05, paired t test. White asterisks represent areas of membrane where the GFP fluorescence disappeared after application of CCh. C, Same experiment as in A but after initial 10 min of CCh, proBDNF (2 ng/ml) was coapplied with CCh for subsequent 10 min. D, Quantification of C (n = 7). E, Same experiment as in C but dishes were pretreated 2 h with a p75NTR function-blocking antibody (1:500). F, Quantification of E (n = 8). **p < 0.01 paired t test. G, Same experiment than in C but the Rac1 inhibitor NSC23766 (50 μm) was bath-applied throughout the experiment. H, Quantification of G (n = 5). Scale bar, 10 μm.

Article Snippet: All drugs were purchased from Sigma-Aldrich, except 6-methyl-2-(phenylethynyl) pyridine, (S)-3,5-dihydroxyphenylglycine hydrate, and NSC23766 from Tocris Bioscience, mBDNF and proBDNF (wild-type mouse, #B240) from Alomone Labs, p75NTR antibody (AB-N01) from Advanced Targeting Systems, and proBDNF antibody (5H8) from Santa Cruz Biotechnology.

Techniques: Transfection, Incubation, Labeling, Fluorescence, Blocking Assay